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The protein sequence similarities were assessed by using the BLASTp programs (http://www.ncbi.nlm.nih.gov/BLAST/; until August 15, 2010).
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To search the WOX genes in Arabidopsis and rice, BLAST was performed using the Blastp program to search the NCBI Non-redundant Protein Sequences Database (http://blast.ncbi.nlm.nih.gov/) using the homeodomain sequence of AtWUS as a BLAST query.
After discarding the fragments corresponding to the primer pair used for the amplification of ws/dgat, clone sequences were translated and compared with reference sequences in databases using the blastp program (Altschul et al. 1990).
The protein sequence was then aligned using the BLASTP program against the CDD database to seek the possible conserved domain [41].
The amino acid sequences were then used to query the non-redundant protein sequence database at NCBI using the blastp program (http://blast.ncbi.nlm.nih.gov/Blast.cgi).nih.gov/Blast.cgi
Homologues of each of the 160 proteins identified in the hamster midbody proteome were retrieved from the nr database at the NCBI using the BLASTp program version 2.2.18 [43].
The P. carinii predicted proteome was compared to 18 complete fungal proteomes listed in Table 4 and to Dictyostelium discoideum proteome, using the blastp program [39] with default parameter values and a Bit-score threshold of 45.
Homologues of the four components of the B. subtilis Bce-modules were retrieved from the 779 complete genomes available in February 2009 at the NCBI using the BLASTp program [36].
The 196 influenza 17 aa peptides were compared using the blastp program against the non-redundant protein sequences database restricted to human (taxid:9606) at NCBI (http://www.ncbi.nlm.nih.gov/BLAST/) to detect the presence of fragments identical to human peptides.
The peptide sequences obtained were also analyzed using the Blastp program.
Every QuartOP was compared to the COG database using the blastp program.
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