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Genotypes of all 278 lines were obtained using the 55k SNP Illumina platform.
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By using the 100K SNP scan of the Framingham Heart Study, we identified an age-varying association between a SNP in ROBO1 and obesity and hypothesized an age-gene interaction.
To quantify the two alleles, measurement of the area under the peak corresponding to each allele was performed using the PSQ™96MA SNP software.
Phylogenetic analysis was implemented in MEGA5 using the 13,390 SNP markers (Tamura and Nei 1993).
A set of 192 BC1 individuals were genotyped using the 384 SNP custom array.
This was confirmed by the haplotype reconstruction using the 1 976 SNP.
We resolved this problem by constructing a maximum-parsimony tree using the 5 SNP, LSP, and MIRU24 repeat numbers.
The present result shows that using the 60 SNP loci, the chance of sampling identical genotypes from a random mating population would be 1 out 100 000.
We estimated genome-wide ancestry for each Kosraen (N=2988) individually using the 106 541 SNP data set (see Materials and Methods section).
By scanning the rapeseed association panel using the 60K SNP array, the clustering results showed that ∼10 000 SNPs had zero call frequency of AA or BB, which could, in part, be caused by inter-homoeologue polymorphisms.
All samples were genotyped by a private company (DNA Landmarks, Inc .. SNP genotyping was performed using the 60K SNP Illumina iSelect™ chicken microarray (Groenen et al. 2011; http://www.illumina.com/).
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