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Background fluorescence was measured using the 3xSLv1 probes.
Background fluorescence was measured using the 3xSLv1 probes; probes with median signal intensities less than the trimmed mean (trim = 5%) plus three trimmed standard deviations of the 3xSLv1 probes were flagged and called absent.
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The background for each array was measured using the 3xSLv1 probe.
The background for the Agilent array was measured using the 3xSLv1 probe, using the hsa_negative_control_1 probes for the Exiqon platform, and using the pool of random hexamers for NCode arrays.
The specificity of the assay using the PA3 probe was investigated with a range of microorganisms, which are commonly isolated from blood culture bottles serving as negative controls.
The objective of this study was to develop a reliable method for the determination of the thermal conductivity of composted material using the TP08 probe.
The optimal amounts of proteins were determined using the P6CON probe as we described elsewhere [15].
To identify the integration site, a genomic library was constructed for tgε26+/+ mice and screened using the Sstr5 probe.
Signals were acquired at a sample rate of 10 Hz from 22 cortical regions on the bilateral prefrontal cortex using the 3×5 probe holder and the corresponding optodes.
Southern blot hybridization was realized on AscI PFGE using the p1-9 previouslyiously described to assess the copy number of SGI1 in tandem arrays.
After transfection (in cycle 0) the endogenous gene was present and in addition two bands of 6 kb and 9 kb, respectively were detected in the blot using the PfLplA1 probe (Fig. 4 B, lane 2).
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