To compare inheritance of the LSE2 and LSE3 phenotype to that of LSE1, segregation analysis was conducted for each LSE mutant line using the M3 generation from stain-negative M2 plants (Table 1).
If genetic screening is not feasible, we suggest propagating the wild progenitor for about ten generations, using only one individual from each generation as a parent for the next, and then using the F10 generation individual to establish an inbred stock of "clonal" individuals.
In this study, we take advantage of BSR-Seq for the analysis of disease resistant genes using the F2 generation backcross progenies (F1 hybrid backcrossed with the susceptible channel catfish) of the interspecific hybrids.
In order to determine the genetic control of SUI and confirm nonlinkage to the S locus, we performed a segregation analysis using the F2 generation between the SUI S -12 (SUI/ SUI, pui/ pui, S / S ) and the non-SUI S (sui/ sui, pui/ pui, S / S ) lines.
During the original screening procedure using the M2 generations, both the two lines segregated into stain-positive and stain-negative plants.
Tissue phenotype characterization was performed using the F1 generations from crosses between dnRAR transgenics and OR-reporter mice, including P2-IRES-tauLacZ, MOL2.3-IRES-GFP-IRES-tauLacZ, and OMP-tauLacZ mice on a C57BL/6 genetic background (7, 27).
We used the F2 generation of the ERE-TG zebrafish derived from line one in all studies reported here.
The LOD thresholds based on the permutation test were very high because we used the F2 generations for QTL analysis.
Restrictive dermopathy was excluded from our list when we used the 13-generation pedigree, because the only known case among the South Dakota Hutterites was not included in the 3657 individuals.
