The neuronal soma was selected in the EGFP stain using the 'freehand selections' tool.
FRAP was measured using FRAP profiler on average projections of confocal stacks, using the freehand selection tool to select the bleached area and normalized to total cellular fluorescence.
To obtain values for the SCV-associated marker in these cells, the bacterial cluster in the best focal plane was selected using the freehand selection tool and the software was then allowed to extend the selection through all z-sections in both channels.
All the bacterial cells in the images were selected individually using the freehand selection tool of the software.
The silicon nanocrystals were marked using the freehand selection tool in ImageJ [17].
For each experimental condition, we traced the outline of the rod spherule, horizontal axonal endings and bipolar cell dendrite using the freehand selection tool (green, Fig. 1A).
Amylase-positive area/pancreas area was measured manually with ImageJ using the freehand selection tool.
Organ measurements were obtained using the Freehand Selection tool to draw ROI around their perimeter and measuring integrated density.
Organ size was quantified using ImageJ: stacks were projected using the function z-stack, a circle was drawn using the freehand selection around each organ viewed from the top and the surface inside this selection was automatically measured.
Preoperative CSDH and postoperative residual rinsing fluid in the haematoma cavity were delineated against soft tissues using the freehand selection tool on each 2.5– to 5-mm tomographic slice of every CT image series by a single experienced neurosurgeon (M.S).
