Different from CS/siRNA and PLA/siRNA polyplexes which had very low transfection efficiency at physiological pH (7.4), CS/PLA/siRNA polyplex formed by using sufficient amount of CS (i.e., 5 : 1 CS/siRNA) together with noncytotoxic level of PLA (i.e., 0.1 : 1 PLA/siRNA) could silence the gene satisfactorily at both pH 7.4 and 6.4, unaffected by the presence of serum.
To this end, we used sufficient amounts of gel and avoided performing sliding movements for as long as possible to minimize shearing force.
Use sufficient amount of white space to break up the text.
In this technique the most important factor for reducing the size of nanoparticles is increase the shear stress during emulsification, which is done by increasing the applied energy, decreasing the polymer content in organic solvent, using the sufficient amount of surfactant, increasing the organic phase volume to aqueous phase volume ratio.
Using a sufficient amount of bisulfite-modified DNA for WGA was critical for downstream application.
First, our analyses revealed that when performing WGA, using a sufficient amount of bisulfite-modified genome DNA is critical.
" Douglas Weaver, head of cardiology at the Henry Ford Hospital in Detroit says: " We've used Zetia without sufficient amounts of clinical data to support it.
The same suspension was ruptured using sufficient volume of ethanol, and the total amount of EGCG was determined spectrophotometrically.
The same suspension was ruptured using sufficient volume of ethanol, and the total amount of Lf was determined spectrophotometrically.
