Sentence examples for using subsequently from inspiring English sources

The yield of the recombinant VEGF165 protein, estimated by SDS-PAGE and Western Blot, was higher when using subsequently lysozyme and sonication treatments, compared to all other methods.

The cross section was polished by using subsequently decreasing beam currents down to 50pA (at 30 kV acceleration voltage).

Using subsequently the "get references" function will return all the relevant pubmed entries in which the gene and keywords co-occur.

RNA hybrids were detected using, subsequently, mouse anti-DIG (1 : 2000, Sigma-Aldrich Chemie GmbH, Steinham, Germany), rabbit anti-mouse Ig (1 : 50, DAKO, Glostrup, Denmark) and mouse alkaline phosphatase anti-alkaline phosphatase (APAAP, DAKO; Hazelbag et al, 2001).

Repetition of in silico extension of EST sequences followed by removal of homologs using subsequently updated databases (April 2006 freezes) removed an additional 134 ESTs, thus leaving 164 putative NTs.

RNA hybrids were detected using subsequently mouse anti-digoxigenin (1 : 2000, Sigma-Aldrich Chemie GmbH, Steinham, Germany), rabbit anti-mouse Ig (1 : 50, DAKO) and mouse alkaline phosphatase anti-alkaline phosphatase (APAAP, DAKO).

The system was working, however, and was used subsequently by firefighters in the south tower".

The next Assumption 3 will be used subsequently.

This fact will be used subsequently in the proof of our main result.

Timing information are withdrawn and only the sequence of recognised chords are used subsequently.

We now recollect some basic definitions, notations, and results that will be used subsequently.