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Testes were removed from pups with fine forceps using sterile procedures and cut into pieces after removing the tunica albuginea.
The muscle was diced into small blocks (2 mm) using sterile procedures and the diced muscle then centrifuged (300 g, 5 min) and the supernatant removed.
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Bats were immobilized face up, and a blood sample was taken using sterile procedures.
All 16 inoculated kestrels and the two uninoculated controls were examined at necropsy when, using sterile procedures, portions of brain, trachea, lung, heart, liver, kidney, adrenal, spleen, duodenum, pancreas, jejunum, cecal tonsil, and cloaca with bursa of Fabricius were collected for histopathology.
Twenty microliters from each well of the source plate was dispensed into each well of recipient 96-well round-bottom polystyrene plates (Corning) using the Evolution P3 Precision Pipetting Platform (PerkinElmer) under a laminar airflow hood using sterile procedures.
Briefly, deep-96-well (VWR) source plates containing 1.3 ml of various TLR ligands at 10 times the desired concentration were prepared using sterile procedures under a laminar airflow hood.
"After all, Dr. Bernstein, don't you use sterile procedure in the US for all surgical interventions?
Prior to behavioral training, each animal was implanted with a custom-made titanium head-post using titanium cranial screws (Gray Matter Research, Bozeman, MT) and an acrylic cranial patch (Palacos Bone Cement; Zimmer Inc., Warsaw, IN) using sterile surgical procedures.
All surgical procedures were performed using sterile techniques and the surgeons used surgical loupes.
All isolation procedures were performed in a laminar flow hood using sterile techniques and supplies.
All operations were performed using sterile techniques and autoclaved equipment.
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