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Total and HDL cholesterol were measured using standardized enzymatic methods (11).
Cholesterol and triglycerides levels were measured from plasma samples using standardized enzymatic methods at Kuopio University Hospital Central Laboratory.
Non-fasting levels of HDL-cholesterol were determined at the Lipid Reference Laboratory of the Erasmus Medical Centre in Rotterdam, using standardized enzymatic methods.
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Total cholesterol was measured using standard enzymatic methods.
Cr was measured based on isotope-dilution mass spectrometry standardized enzymatic method using the SRM 967 as control.
Serum creatinine and ScysC were both measured using standardized methods, IDMS-traceable enzymatic method (Crea Vitros) and IFCC-traceable N Latex cystatin kit (Siemens), respectively.
Height and weight were determined using standardized conventional methods.
Plasma glucose and A1C were tested at the University of Missouri Diabetes Diagnostic Laboratory using standardized NHANES methods (11).
The AES was translated into Chinese and validated using standardized psychometric methods.
Carotid artery intima-media thickness (cIMT) and elasticity were measured using standardized methods.
Natural selection was measured using standardized methods developed by Lande and Arnold (1983).
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