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Effect of roughness on the accuracy of the measurement was tested by using standard targets of known roughness values.
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The relative transcript amount of the target gene, calculated using standard curves of serial cDNA dilutions, was normalized to that of β-actin of the same cDNA.
These approaches could be explored by targeting genes presented in this study using standard methods of cotton transformation.
The parameters that affect the extraction of the target analytes was optimized using standard reference material of San Joaquin soil (SRM 2709a).
To date, there has been no study using standard DBT to specifically target EDs characterized by problems of OC.
This was calculated using standard curves from serial dilutions of target template.
After the PCR had been completed, the LightCycler software (Roche Diagnostics, Mannheim, Germany) automatically converted the raw data into copies of target molecules using standard curves.
In previous works [15, 16, 47, 48], target classification algorithms using standard sidescan sonars have mainly been based on the analysis of the targets' shadows.
Absolute quantification was carried out using standard curves generated by serial dilution of target amplicon-containing plasmids (pGEM-T easy, Promega, Annandale, NSW, Australia), to cover up to 5 logs of amplicon copy number per microlitre.
Relative expression of target genes was calculated using standard curve method and normalized to HPRT mRNA content.
A few targets were further confirmed using standard compounds.
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