Sentence examples for using standard suspensions of from inspiring English sources
Calibration of the DLS spectrometer was conducted using standard suspensions of monodisperse latex microspheres (Polysciences, PA, USA).
The cell suspension was further diluted as required using standard media to prepare a stock cell suspension of 50,000 cells/mL.
Protoplasts were prepared from 3 d old tobacco BY-2 suspension cells using standard methods [ 52, 53].
Primary retinal cell suspensions were generated using standard methods [ 1- 3].
Having low cytoplasm/nucleus ratios like lymphocytes or suspension cells of tumor cell lines, transfection efficiencies using standard nucleofection conditions for total PBMC preparations were found to be in the range of 30 70% of all cells [26].
All FACS studies were performed using single cell suspensions, and cells were stained using standard protocols.
Single-cell suspensions of BM cells, splenocytes or PB cells were stained using standard procedures.
Following which standard 0.5 McFarland bacterial suspension is prepared in TSB for use in the study using standard protocols [ 20].
T cells were purified from LN cell suspensions by removal of B cells with anti-Ig-coated Dynabeads (Invitrogen, Carlsbad, CA) using standard procedures.
The pH value of the ZnS suspension before centrifugation was recorded using a combined glass electrode, which was calibrated using standard pH buffer solutions before use.
Following incubation, a suspension of C. jejuni in MEM was added to each well and binding and internalization assays were performed using standard laboratory protocols.
