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After the optimisation of the methodology using standard solutions, as a practical analytical application, three food matrices (poultry, eggs and milk) spiked at three different dioxin levels were analysed.
A standard curve was generated by using standard solutions as per manufacturer's instructions.
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Quality was controlled using standard solutions.
P4HB is a thermoplastic material that can be processed using standard plastics processing techniques, such as solution casting or melt extrusion.
Myocytes were isolated as previously described [40] using standard intra- and extracellular solutions for AP recordings (see [40] for details).
Proteins, i.e., BSA, EA, pepsin, and trypsin, were individually dissolved (0.1 wt%) in the phosphate buffer (0.05 M, pH 7.2) and used as standard solutions.
Hydroxyethyl starches (HESs) have been used as standard solutions for volume replacement therapy for decades.
Lactic acid (20 mM), succinic acid (10 mM), and fumaric acid (10 mM) were used as standard solutions to identify the peaks.
Acetic acid (20 mM), propionic acid (10 mM), butyric acid (10 mM), isobutyric acid (10 mM), valeric acid (10 mM), isovaleric acid (10 mM), and 4-methylvaleric acid (10 mM) were used as standard solutions to identify the peaks.
Calculate exact concentrations of each component in stock standard solution as shown below: Stock standard A use concentration as is.
After addition of 10 ml deionized water, the absorbance of the final solution at 540 nm was measured against DNS reagent/water blank using Visible Spectrophotometer (DU7400, Beckman Coulter, Inc .. Aqueous glucose was used as standard solution to obtain a calibration line.
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