Sentence examples for using standard protocols as from inspiring English sources

Human miRNA genes were cloned into LeGO-vector derivatives and lentiviral supernatant was generated and collected using standard protocols as previously described64.

Genomic DNA from each strain was isolated using standard protocols as previously described [19].

The preparation of yeast genomic DNA, ethanol lysates, SDS-PAGE and immunoblotting were performed using standard protocols as described earlier [33], [34].

Western blot analysis was performed against parasite proteins resolved on a 12% SDS-PAGE under denaturing conditions using standard protocols as described elsewhere [26].

Sequencing, assembly, finishing, pipeline annotation, and verification were completed by staff members of the US Department of Energy Joint Genome Institute (JGI, Walnut Creek, CA, USA) using standard protocols as published online (http://www.jgi.doe.gov/sequencing/protocols/prots_production.html).html

The MSIA-Tips utilized here were prepared using standard protocols as previously described [18], [20], but tailored with a RBP4/beta-lactoglobulin antibody ratio of 16.4∶1 (wt/wt), respectively.

Anthocyanin content was determined using standard protocol as described in Fankhauser and Casal (2004).

Immunohistochemistry analysis was performed using standard protocol as the manufacturer recommended (EnVision System; Dako, Carpinteria, CA, USA).

BAC culture and sequencing reactions were conducted using standard protocols, and as previously described [ 25, 40].

TaqI restriction enzyme digestion was carried out on genomic DNA extracted from leaves using standard protocols [50] with modifications as in [51].

Primary human keratinocytes, dermal fibroblasts and melanocytes were isolated from healthy skin using standard protocols and cultured as described [21], [22].