Sentence examples for using standard procedures as from inspiring English sources
Yeast strains are listed in Table 1 and plasmids are listed in Table 2. Yeast strains were grown using standard procedures as well as methods published previously (Leon et al. 2008).
Bicarbonate (HCO3 −) was determined by titration method using standard procedures as described in AHPA manual (APHA 2005).
Immunohistochemistry on vibratome slices of paraformaldehyde-fixed tissue was performed using standard procedures as described [29], [49] and imaged using confocal laser scanning microscopy.
Proteins were isolated from myotubes using a protein lysis buffer and Western blot analysis was carried out using standard procedures as previously described [16].
After 30 minutes the tension of the aortic segments and mesenteric arteries were normalised to a tension equivalent to that generated at 90% of the diameter of the vessel at 100 mmHg, using standard procedures as described previously [15].
All patients were screened for DNA-mutations in the following genes: SOD1, vesicle associated membrane protein B, TDP-43, fused in sarcoma (FUS), progranulin, chromatin modifying protein 2B, optineurin, ataxin 2 and angiogenin using standard procedures as described [15], [16], [17], [18], [19], [20], [21], [22], [23].
Cell culture and transfections were performed using standard procedure as described previously [ 38].
Immunohistochemistry used standard procedures, as described in our previous studies (Donskov et al, 2002a, 2004a).
No alternative splice transcript has been identified in the region comprising the c.7041+8G/A variant using standard procedures such as PCR amplification with external primers located on exon 38 39, 39 40, 43 44 and 44 45 junctions, followed by subcloning.
For these purposes we compared the development of an array of biochemical parameters measured in blood plasma using standard procedures of dry chemistry as well as electrochemical devices following a subcutaneous infection with a wild Francisella tularensis subsp. holarctica strain (a single dose of 2.6 × 109 CFU pro toto).
GST proteins were purified using standard procedures and precipitations were performed essentially as previously described [ 25].
