Sentence examples for using standard primers and from inspiring English sources

The amplicon (GenBank accession no. JN800509) was sequenced by using standard primers and protocols (10 ).

New sequences were obtained using standard primers and protocols available at http://pubmlst.org/wolbachia/wsp/info/protocol.shtml.shtml

Because of this complementarity, over 98% success in recovery of a barcode amplicon can be anticipated across all Hexapoda by two PCR reactions, the first using standard primers and the second (for those that failed in the first reaction) employing the primer set developed in this study.

Positive colonies were selected on LB agar with Kanamycin and tested for the presence of yqi using standard primers IMT-P1560 IMT-P15611561 (Table 4).

Positive clones were selected on LB agar with Kanamycin and tested for the presence of the 4,975 bp gene cluster using standard primers IMT-P1560 IMT-P15611561.

Here we use single-locus approaches to evaluate the status of Rhithrogena species and species groups using standard primers for one mtDNA marker (cox1) and newly developed primers for one nDNA marker (PEPCK).

Briefly, fragments between 500 and 1,500 bp were amplified using standard primers (Simon et al. 2006, Supplemental Primer List) and sequenced on both strands using the amplification primers.

The ompB gene was amplified from clinical samples and DNA by using standard primers (18 ), except for the ultimate forward primer 120-M59, wasch was found to produce nonspecific amplicons with the human DNA in clinical samples.

The general strategy for amplification and sequencing was to amplify fragments of 500 1,500 bp using standard primers (Simon et al. 2006).

Additionally, these primers were characterized by a 5′ extension that allowed for reamplification using standard primers after the first amplification employing the specific primers.

In 31/79 integron positive E. coli strains, the gene cassette regions could not be PCR amplified using standard primers.