Sentence examples for using standard molecular techniques from inspiring English sources

DNA manipulation, enzymatic digests, ligation and PCR reactions were performed using standard molecular techniques (Sambrook and Russell, [1989]).

Genomic DNA was isolated and prepared using standard molecular techniques.

Southern blot with 10 µg of genomic DNA, previously digested with BamHI, was performed using standard molecular techniques.

The VTI13 promoter was inserted upstream of the GFP VTI13 construct using standard molecular techniques.

Gels were then Blotted using standard molecular techniques and the resultant nitrocellulose membranes (Whatman) were blocked in 5% milk in TBS 0.1% Tween 20 (TBST).

Using standard molecular techniques, the resultant PCR product was inserted into the BamHI/ XhoI sites of pcDNA3 to yield hG6-MYQ.

All RNC constructs were prepared using standard molecular cloning techniques and cloned in the pBAD vector (Invitrogen) using the NcoI and HindIII restriction sites, resulting in an additional glycine after the initiator methionine.

All molecular methods were performed using standard molecular biology techniques (Sambrook et al. 1989).

Lentiviral constructs were generated using standard molecular biology techniques (Figure 1).

Plasmids were constructed using standard molecular biological techniques [74].

All plasmids were constructed using standard molecular cloning techniques [37].