Sentence examples for using standard molecular procedures from inspiring English sources

Plasmid DNA purification, PCR amplification of genes, isolation of fragments from agarose gels, cloning, restriction enzyme digestion and T4 DNA ligation were performed using standard molecular procedures [ 76].

Lentiviral particles were generated using standard molecular biology procedures [ 22].

The vector for the U2 snRNA overexpression was generated using standard molecular cloning procedures.

Cloning was performed using standard molecular biology procedures and verified by sequencing.

Cloning was performed using standard molecular biology procedures and verified by DNA sequencing.

The YFP STIM1 NN) mutant was constructed using standard molecular biology procedures and based on the construct described previously [ 22].

After confirmation of the mutations by DNA sequencing, the precursor was placed downstream of the UBQ10 promoter in a binary vector using standard molecular biology procedures.

Plasmids were constructed using standard molecular biology procedures and they were purified with the Qiagen plasmid Maxi Kit (Qiagen, Valencia, CA).

DNA sequences encoding ZFNs of choice (Supporting Information, Figure S1 and Table S1) were assembled by mutagenesis and overlapping polymerase chain reaction (PCR) using standard molecular cloning procedures.

pACT-TCTEX1D4 – TcDNA1D4 cDNA was digested from Myc-TCTEX1D4 with EcoRI /XhoI, and inserted into EcoRI/ XhoI digested pACT-2 (Clontech, Saint Germain-en-Laye Germain-en-Laye standard molecular biology procedures.

Murine K2P channels were cloned into pGEMHE/pMO, IRES-GFP (Invitrogen), or pYES2-MET25 (high copy 2 μ, URA3) for expression in oocytes, HEK-293T cells, and yeast, respectively, using standard molecular biology procedures, and verified by DNA sequencing.