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Specimens were immediately delivered to the laboratory and quantitatively processed for bacterial and fungal cultures using standard methods, as have been described previously [ 18].
Nucleic acid manipulations not described earlier were performed using standard methods, as described by Sambrook et al. A hierarchical cluster analysis of culture conditions was performed with the nearest neighbor-joining method, with STATISTICA software (StatSoft, Inc ., in which the normalized frequency values of the contigs were used as variations.
This was achieved using standard methods as suggested by Rodier (1996).
Rhizobia and PSM inoculum were prepared using standard methods as described above and were applied in all the treatments.
The inoculum preparation and seed inoculation were performed using standard methods as described earlier in the above paragraph.
Subsequently, the samples were analyzed in the laboratory for their chemical constituents such as Ca2+, Mg2+, Na+, K+, Cl−, SO42−, HCO3− and NO3−, using standard methods as suggested by the American Public Health Association (APHA 1989, 1995a, 1995b).
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The cluster analysis was performed using standard method as Squared Euclidean Distance and Ward's linkage method (Singh et al. 2015).
Sample characterization was performed using standard methods such as XRD, SEM, TEM, impedance spectroscopy and optical reflectometry.
Protein lysates from aliquots of cells prepared for ROS measurement were analysed for Nbn exon6 deletion efficiency by immunoblotting using standard methods and as described before [43].
This allows shape variation to be explored using standard methods such as principal components.
Analysis of genome-wide data is often carried out using standard methods such as differential expression analysis, clustering analysis and heatmaps.
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