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To determine state 4 and state 3 respiration, oxygen consumption was continuously recorded with sequential additions of 5 mM succinate, 0.2 mM ADP, and finally 2.5 µM carbonyl cyanide p-[trifluoromethoxy]-phenyl-hydrazone (FCCP) to induce maximal chemical uncoupling using standard methodology as we have employed in the past [30].
The metabolite analyses were performed using standard methodology as described previously [ 23], with some minor modifications as described here.
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Structure Determination, Model Building, and Structure Refinement The eIF3a, eIF3b β propeller and eIF3b-CTD/eIF3i/eIF3g-NTD structures were solved and built using standard methodologies as described in Experimental Procedures.
The eIF3a, eIF3b β propeller and eIF3b-CTD/eIF3i/eIF3g-NTD structures were solved and built using standard methodologies as described in Experimental Procedures.
The secondary swine anti-rabbit horseradish peroxidase (HRP -conjugated antibody, as well as nonimmune rabbit serum were obtained from DAKO (Cambridge, UK) ImmunoHRP -conjugatedtantibodyor caseolin-1 wellcasried out usinonimmunerabbithodologieserumpreviously described (Campbell et al, 2002).
For most equipment, scaling factors were applied for variations in the throughput or other key design parameters relative to the original design basis using standard methodologies as described in the NREL 2002 and 2011 design cases [ 1, 18].
Skin testing was performed using standard methodology.
Growth parameters were derived using standard methodology.
Testing was performed using standard methodology [12], [13] and reference equations [14].
were also collected using standard methodology.
DNA was extracted using standard Methodology.
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