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The eIF3a, eIF3b β propeller and eIF3b-CTD/eIF3i/eIF3g-NTD structures were solved and built using standard methodologies as described in Experimental Procedures.
For most equipment, scaling factors were applied for variations in the throughput or other key design parameters relative to the original design basis using standard methodologies as described in the NREL 2002 and 2011 design cases [ 1, 18].
The secondary swine anti-rabbit horseradish peroxidase (HRP -conjugated antibody, as well as nonimmune rabbit serum were obtained from DAKO (Cambridge, UK) ImmunoHRP -conjugatedtantibodyor caseolin-1 wellcasried out usinonimmunerabbithodologieserumpreviously described (Campbell et al, 2002).
Structure Determination, Model Building, and Structure Refinement The eIF3a, eIF3b β propeller and eIF3b-CTD/eIF3i/eIF3g-NTD structures were solved and built using standard methodologies as described in Experimental Procedures.
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To determine state 4 and state 3 respiration, oxygen consumption was continuously recorded with sequential additions of 5 mM succinate, 0.2 mM ADP, and finally 2.5 µM carbonyl cyanide p-[trifluoromethoxy]-phenyl-hydrazone (FCCP) to induce maximal chemical uncoupling using standard methodology as we have employed in the past [30].
The metabolite analyses were performed using standard methodology as described previously [ 23], with some minor modifications as described here.
The results presented raise important questions regarding the need of using standard methodologies in dynamic model construction as well as in using experimental data for model validation.
Typing of bacterial targets will be performed, as appropriate, using standard methodologies [ 55] to evaluate the ability of our surveillance and comprehensive diagnostic approach to discover outbreaks not identified by traditional approaches.
We found minimal evidence of publication bias for pneumonia using standard methodologies.
A cDNA library was constructed from adult tissue of the acoel Convoluta pulchra using standard methodologies.
As a consequence of its autolysis, analysis of p94 protease activity using standard methodologies is difficult.
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