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COVS was performed by each patient using standard instructions.
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The patients were instructed by a pain specialist nurse using standard French language instructions on how to use the Faces Pain Scale - Revised [ 13] as shown at http://www.painsourcebook.ca.ca
The Jamar dynamometer is considered to be a reliable and valid instrument for measuring handgrip strength when calibrated and using standard positioning and instructions [ 10].
Genomic DNA was extracted using standard procedures, following instructions from the Aqua Pure Genomic DNA Isolation kit (Bio-Rad) and Wizard Genomic DNA Purification kit (Promega).
ELISA was performed precisely according to manufacturer's instructions using standard kits (Cellestis).
CD3+, CD4+ and CD8+ were measured by flow cytometry (FACSCount, BD) according to the manufacturer's instructions using standard reagents (BD).
CK determination was performed according to the manufacturer's instructions using standard spectrophotometric method with enzyme-coupled assay reagent from Fisher Scientific (CK10).[30] Absorption at 340 nm was measured every min for 2 min at 37°C to calculate enzyme activity.
No later than 24 hr after the blood draw, the lymphocytes collected in CPTs containing sodium citrate were isolated according to the manufacturer's instructions using standard buoyant density centrifugation methods.
No later than 24 hr after the blood draw, lymphocytes collected in CPTs containing sodium citrate were isolated according to the manufacturer's instructions using standard buoyant density centrifugation methods.
A standard curve was generated by using standard solutions as per manufacturer's instructions.
All biochemical laboratory tests were performed in Burlo clinical laboratories by using standard commercial kits and following manufacturers' instructions.
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