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Double mutants were made using standard genetic methods, and the genotypes were confirmed using PCR or PCR followed by sequencing.
Yeast deletion strains were made by insertion of the KanMX and/or NatMX cassette in the CEN.PK113-7D CEN.PK113-7D 34] using standard genetic methods (Tabackground
For the UNC-63::YFP experiments, fer-1 mutants were crossed into the unc-63::YFP strain (Gendrel et al., 2009) using standard genetic methods.
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We used standard genetic methods and S. pombe media for vegetative growth (EMM and YES).
Using standard genetic mapping methods (see Materials and Methods) we found that the LSJ1 early egg laying phenotype was linked to LSJ1 DNA on chromosomes II and V (Figure S3a).
Studies were done in Saccharomyces cerevisiae (W303 background) using standard genetic and molecular methods.
Strains were constructed using standard genetic techniques.
Crosses were performed using standard genetic techniques.
Double mutants were constructed using standard genetic techniques.
Double and triple mutants were generated using standard genetic techniques.
The other fly strains used were generated by standard genetic methods using individual mutant and transgenic fly lines described above.
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