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The glow that surrounds the stereogram image derives from the fact that this view of the hook is assembled using standard confocal software and the viewer is looking through preceding and following images which are a result of the perspective of looking at angled images.
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Images were viewed using standard SenseCam software.
Images were obtained using Leica confocal software (version 2.61).
Images were obtained by confocal microscopy (Leika TCS SP2 AOBS; lens 63x) and analyzed using Leika confocal software.
Semi-quantification was performed using Leica confocal software.
Image acquisition and processing were carried out using the Leica Confocal Software (Leica Lasertechnik).
Images were processed using the Leica Confocal Software (Leica Microsystems) and Adobe Photoshop 7.0.
Images were acquired with Leica TCS SL confocal system using the Leica confocal software (Leica Microsystems, Wetzlar, Germany).
Post-capture image analysis and processing of confocal image stacks were performed using the Leica Confocal software.
Images were acquired with a Leica TCS SP2 confocal system (Leica Microsystems Heidelberg, Mannheim, Germany) using the Leica Confocal Software (LCS).
Fluorescent confocal laser scanning microscopy was conducted with a Leica SP2 MP laser scanning confocal microscope using the Leica confocal software LCS Lite Leica Microsystems HD GmbHH, Mannheim, Baden-Württemberg, Germany).
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