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The relative change in RIM1 transcript between experimental and control tissue was normalized to MAP2 transcript levels using standard calculation methods (Pfaffl 2001).
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The mean ± standard error was calculated using standard calculations available in the OriginPro spreadsheet.
For our case and using standard calculations they are: 97, 97.5, and 98%.
The mean (bar heights in Fig. 2) and standard deviation (error bars in Fig. 2) were determined using standard calculations.
The microhardness of each sample was evaluated using standard calculations [ 15].
In a number of cases where standard errors, confidence intervals (CIs) or CMRs were not reported, these were estimated using standard calculations with data that were provided.
One study compared APACHE II, APACHE III and SAPS II scores using standard calculations, and then taking into account pre-ICU data [ 32].
Using standard calculations for repeated significance tests (11) with 10 equally spaced looks and 62.5% information at the 10th look, the probability that a single outcome would have P ≤ 0.04 at one of these looks is 0.16.
The suitability of the new model is verified by regression analysis in comparison with the commonly used standard calculations.
In fact, when the functional variables are explicitly modelled, linear regression has >80% power to detect the gene-gene interaction effects simulated here (n = 2000, sr main = 0.01, sr interaction = 0.05), determined using standard power calculation techniques for gene-gene interaction [ 70, 71].
Uncertainty of the predictions was determined using standard error calculations on the two modeling parameters for water flow decline rates.
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