All of these isolates were submitted to the National Tuberculosis Institute, where they were identified as M. abscessus using standard biochemical methods and rpoB PRA.
Demographic data were collected and a blood sample was taken to assess lipid profile, CRP, serum creatinine, calcium and phosphate using standard biochemical methods [ 7].
Mice were observed for 7 days and lungs were isolated on designated days for studying difference in bacterial load and other proinflammatory mediators using standard biochemical methods and ELISA.
The isolates were obtained from different clinical samples (wound swab, urine, pus, ear swab, blood and vagina swab) and were verified using standard biochemical methods as described previously [ 16].
Equal amounts of protein were prepared using standard biochemical methods (described in detail in Supplementary material online, Methods) and subjected to sodium dodecyl sulfate polyacrylamide gel electrophoresis and electrotransfer to Immobilon-P membranes (Millipore).
Lactose-fermenting colonies were identified as E. coli by using standard biochemical methods, which included sulfide indole motility, growth on triple sugar iron medium, oxidase activity testing, and the API20E system (bioMérieux, Durham, NC, USA) as needed.
Investigations on treatment of ZnO nanoparticles on Staphylococcus aureus MTCC 737 strain was essentially made by using standard biochemical method.
A total of 137 presumptive Enterococcus isolates were identified to species level using standard biochemical tests and analyzed for resistance to erythromycin, clindamycin, virginiamycin, tetracycline, and vancomycin using the agar dilution method.
Presumptively, the organism was identified as M. fortuitum using standard biochemical reactions like nitrate reduction and 68 °C catalase.
Using standard biochemical protein-protein interaction assays, we found that Wise can also bind to Lrp4.
PTH-dependent anabolic activity was evaluated in these mice using standard biochemical and histomorphometric criteria.
