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Best production performance was attained when cells were transfected at mid-log phase (2 3 × 106 cells/mL) with medium exchange at the time of transfection using standard amounts of plasmid DNA and polyethylenimine.
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For quality purpose Proper thin wet mount and Kato-Katz techniques was prepared using standard amount of specimen and examined within a given time.
In a study conducted by Dr. Ettinger, 138 women who had been using the standard amount of H.R.T. for an average of nine years were switched to a half-strength dose of estrogen and were given progestin less frequently (for 14 days once every six months rather than monthly).
Specificity of each of the mouse PYHIN primer sets was assessed by running qPCR using a standard amount of each of the various different cloned PYHIN cDNAs as templates.
Western blotting was performed using standard procedures, and equal amounts of total protein were electrophoresed on 4 20% Tris-Glycine gels and MagicMark (Invitrogen, Carlsbad, CA, USA).
Over 1m gallons of these toxic chemicals have been dumped in the Gulf thus far.2 32pm: Asked about his thoughts on the decision not to use the standard amount of concrete during drilling, Mr Hayward responded, "I'm not the drilling engineer, so I'm not qualified to make those judgments".
But Facebook tells me it feels confident that Live Location uses a standard amount of battery, and Marcus writes "We put a lot of time and effort into … minimizing battery consumption to the point you wouldn't even have to think about it". Though it's still worth keeping an eye on it.
But Facebook tells me it feels confident that Live Location uses a standard amount of battery, and Marcus writes "We put a lot of time and effort into … minimizing battery consumption to the point you wouldn't even have to think about it".
Yields of total RNA for PBMC samples were generally low and for the majority of patients it was not possible to use the standard amount of total RNA (≥ 5 μg) as recommended in the standard protocol.
The system was calibrated using standards containing known amounts of 5-HT, NandnDADA.
The ratio of α:β in 1 1 mixtures was estimated by densitometry using standard curves prepared from known amounts of NrdE and NrdF as described previously.
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