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Further, the quality of the generated data has been compared with published data and is superior to that obtained using spotted oligonucleotide microarrays.
This corroborates the findings of a smaller scale diagnostic assay using spotted microarrays of Tm-matched probes immobilized on agarose coated slides [20], [22], as well as other studies [6], [13], [19], [23] [29].
To assess the effect of Sirt1 deficiency on spermatogenesis in more detail, we compared global gene expression for Sirt1−/− and Sirt1+/− testis using spotted oligonucleotide microarray technology (all analyses were performed using the MEEBO oligonucleotide library- microarrays were purchased from Stanford University microarray facility).
Samples were analyzed before and up to 1 hour after the shock using spotted microarrays.
It was fabricated using spotted oligo technology, which limits the number of probes that can be included on one array.
Comprehensive transcript profiling was carried out using spotted cDNA microarrays containing 44,000 spots representing approximately 27,290 unique Unigene clusters as described.
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For example: try using "spot the difference" pictures.
If it is difficult to see your baton, try using spotting tape, or electrical tape, on your baton.
We used spotted membrane DNA array screening to identify gene expression changes associated with FBN1 mutations.
For this, we used spotted DNA microarrays containing probes for the complete gene set of the S288C reference strain.
The Android camera app offered the option of using spot metering so the light balance was better.
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