It shows the ability of Raman spectroscopy to detect the formation of hydroxyapatite layer at the surface of bioactive sol-gel glasses and also presents a brief description of using Raman spectroscopy to monitor the degradation of polymeric scaffolds.
The temperature-induced unfolding of FBP28 and the tryptophan mutants was studied over the range from 5to8585 °C in 5 °C intervals using FTIR spectroscopy to monitor the amide I′ region.
Here we used Raman spectroscopy to monitor (in terms of glycoprotein yield prediction) a fed-batch fermentation from start to finish.
We use NMR spectroscopy to monitor ligand content of human and Escherichia coli DHFR (ecDHFR), which bind different co-purifying ligands during expression in bacteria.
We also used localized spectroscopy to monitor the uptake of diminazene acturate, an antitrypanosomal agent, into compartments of a single living oocyte.
Accordingly, we used UV spectroscopy to monitor the CTAB content; properly adjust the rotational speed and the number of centrifugation stages; and design a method that can effectively remove impurities, avoid clustering, and enable particle size measurement.
Furthermore, we also use light-scattering spectroscopy to monitor changes in thylakoid membrane structure depending on the protein phosphorylation state and varying concentrations of MgCl2, a salt important in maintaining grana structures in isolated thylakoid membranes [9], [10].
In addition to H NMR spectroscopy, we also used inline IR spectroscopy to monitor the conversion of the alkoxyalkyne to the amide.
Simonsen et al. used near-infrared spectroscopy to monitor the redox state of the terminal element of the mitochondrial respiratory chain, cytochrome a,a3, in skeletal muscle cells of baboons rendered septic by an infusion of viable Escherichia coli [ 15].
This manuscript presents a systematic framework of using Near Infrared (NIR) spectroscopy to monitor moisture content in an alumina mulling process that is commonly used in the upstream operation of catalyst supports production.
