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Quantitative real-time-PCR was performed applying LightCycler technology (Roche) [10] using specific sets of QuantiTect Primer Assays according to the manufacturer's instructions (Qiagen, Hilden, Germany).
Three azo-reductase genes were amplified from genomic DNA of P. aeruginosa (isolate B1) by PCR using specific sets of primers.
In conventional information systems, the majority of text information retrieval and discovery methods are based on using specific sets of pre-defined document metadata, e.g. keywords or indexes of terms characterizing the texts content.
Using specific sets of cell surface markers, these cells can also be isolated from murine and human lung and tested in 3D culture systems and in vivo transplant assays.
To date, three dominant types of cellulase systems have been identified, each using specific sets of enzymatic synergisms [ 2].
We performed real-time PCR (TaqMan, Applied Biosystem, Carlsbad, CA) as described previously (Crespillo et al., 2011a; Decara et al., 2012) using specific sets of primer probes (supplementary material Tables S2, S3).
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However, despite using specific set of primers, we could not amplify the third bovine endopin 2, named 2C [ 36] on our four different BAC DNAs.
By using specific set of primers (6 ), a 350-bp miniexon gene fragment was successfully recovered by PCR; the fragment corresponded to the T. cruzi I lineage, according to miniexon gene typing assay (6 ).
Most of these studies use specific sets of coregulated genes to identify common predicted TFBS clusters, and therefore cannot be applied directly to the study of general properties of promoters.
Further, every application is site dependent and uses specific set of indicators.
More specific modules are most likely to be found by applying our method using different, specific sets of experiments.
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