Eighteen used specific protocols for the weaning from ECMO.
Electronic devices communicate using specific communications protocols.
Hemagglutination and Hemagglutination Inhibition (HAI) tests were performed (detection and typing of viruses) using specific antisera, as recommended by WHO standard protocols [ 17].
Cells were washed in ice cold phosphate-buffered saline containing 125 mM glycine, 1 mg/ml Pefabloc, 1 μg/ml aprotinin and 1 μg/ml pepstatin A. Chromatin was sonicated and immunoprecipitated using specific antibodies, as described in the ChIP protocol from Upstate Inc. (Charlottesville, VA).
AKT, ERK, and their phosphorylated forms, as well as PI3Kγ p110 were detected and quantitated in Western blots using specific antibodies (Cell Signaling Technology) following the protocols of reagent kits.
Expression of GRKs and dominant-negative GRKs (see below) was determined using standard immunoblotting protocols as described previously using specific anti-rabbit polyclonal antibodies against GRK2, GRK3, GRK5, and GRK6.
Expression of 250 miRNAs was analyzed using specific primers and TaqMan probe for each miRNA according to the TaqMan MicroRNA Assay protocol, as previously described [62].
Based on these findings, the purpose of this study was to verify whether REAC technology, using a specific protocol known as the "in vitro regenerative treatment protocol" (IVRTP), may be able to influence the in vitro production of beta-galactosidase from human adipose-derived stem cells which have been subjected to an aging process throughout multiple passages in culture.
Even in the prospective part we completely left the preferred approach of diagnosing and the sequence of measurements to the pneumologists and did not ask to follow a specific protocol, as commonly used e.g. in multicenter clinical trials.
The MA-plot scatterplots did not display any striking differences as most of the technical biases were reduced by using automated protocol as well as specific slides for reducing differences due to dye incorporation.
