Sentence examples for using specific probes and from inspiring English sources
miR-214mut could be distinguished from miR-214 by using specific probes and primers in qRT-PCR assay.
> We experimentally verified the presence and absence of miR1511 by Northern blotting using specific probes and U6 snRNA as an internal reference.
cDNA was synthesized by reverse transcription using the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems) and subjected to real-time PCR using specific probes and reagents from Applied Biosystems.
We quantified the expression levels of a variety of anti-apoptotic Bcl-2-family members and IP3R isoforms in both SU-DHL-4 and OCI-LY-1 at the mRNA level using specific probes, and at the protein level using specific and validated antibodies.
We used specific probes and primers for Human HPRT 1 (hypoxanthine phosphoribosyltransferase 1) as reference gene (Human HPRT1 Hs99999909_m1, ABI).
To address these concerns, later adaptations of the survey that were piloted used specific probes and examples to obtain more specific details [unpublished data, 2013].
We tested recovered strains with the Accuprobe system using specific probes for MAC and MTB.
Hybridization experiments using specific probes for tps10 and tps23 showed that both gene transcripts accumulated in the whole leaf after herbivore damage, but could barely be detected in the epidermis.
Four μL of neat cDNA was amplified using specific probes for Nestin (NC_000001.10) and GUSB (NM_000181.3) in the RT-PCR mix (TaqMan Gene Expression Assay, Life Technologies, respective ID assay: Hs04187831_g1, Hs00939627_m1).
Amplification products were checked by dot blot or Southern blot hybridisation (Stanta and Bonin, 1998) using specific probes: 5′-CCCTCCCGGAGATAAAACCGCCGG-3′ for EEF1A2 and 5′-CGGGTGGTAAACTCCATCTAAGGC-3′ for 28S rRNA.
The FISH analysis was performed using specific probes corresponding to the h-prune and cyclinD1 genomic regions at chromosomes 1q21 and 11q13 (respectively) as well as control clones spanning the peri-centromeric regions at chromosomes 1 and 11, respectively.
