Sentence examples for using specific primers of from inspiring English sources
Products of 573, 596 and 766 bp were detected using specific primers of plnA, plnEF and bre174A, respectively.
The presence of plnEF gene in all identified Lactobacillus plantarum strains was confirmed, which correspond with the results of Chaalel et al. (2015) based on the observation of the PCR product using specific primers of plnEF in Lactobacillus plantarum LbM2a.
The results of PCR analysis using specific primers of genes encoding Bacteriocin, revealed the presence of Plantaricin A and Plantaricin EF in all Lactobacillus plantarum isolates and Brevicin 174A in 5 of Lactobacillus brevis isolates, whereas the gene encoding Pediocin PA-1 was not observed in any of examined isolates.
Absence of virus transcription was controlled by RT-PCR using specific primers of the viral innexin-1 gene [ 48].
The absence of contaminant DNA in the RNA samples was verified by PCR using specific primers of a known gene and gel electrophoresis analysis.
Furthermore, using specific primers of the M. oryzae alcohol oxidase gene, we detected the alcohol oxidase gene in conidia/mycelia that was induced and purified from healthy rhizome tissues, indicating that M. oryzae was present in red rice rhizomes.
PCR amplification that used specific primers of this rep gene also gave positive results from whole-cell DNA of E. coli (p42) transformant, indicating that p42 also belonged to IncU-type plasmid family.
Using specific primers for each of the three zebrafish ERs the expression levels were quantified using real time PCR methodology.
Using specific primers for the amplification of exfoliative toxins, we found that HBXX06 carried Exh-C in its genome DNA (Figure 2C).
RT-PCR was performed using specific primers for TDFs proportionally representative of each functional category.
This technique is based on a modification of RT-PCR methods using specific primers, one of which was localised in E7 region of viral genome and a second one which contains specific adapter sequences joined with oligo dT) [ 2, 3].
