Sentence examples for using specific primer sets from inspiring English sources

Five miRNAs were selected for qRT-PCR analysis using specific primer sets to isolate and amplify mature miRNAs.

The 5′- and 3′-flanking regions of poxB were PCR-amplified from genomic DNA from strain MG1655 using specific primer sets: sSN1185/sSN1186 and sSN1187/sSN1188, respectively.

A total of 114 Campylobacter isolates from 572 intestinal samples were all identified as C. coli via both classical methods and molecular methods, including 16S rDNA sequence analysis and polymerase chain reactions (PCR) using specific primer sets for the hippuricase gene and the aspartokinase gene, designed to differentiate C. coli from C. jejuni.

RT-PCR was performed using specific primer sets (Table S5).

Amplifications were done using specific primer sets flanking the putative Lef/Tcf motifs in the MMP-14 promoter.

PCR amplifications were then performed to test for presence/absence of Wolbachia using specific primer sets for the wsp gene [31] and conditions as previously described [27].

MfdD778A having point mutation in Walker B motif of the ATPase domain was generated by mega primer method, using specific primer set WbF-WbM whereas WbF-WbR primer set was used for screening and confirmation of mutation.

A MtbMfd having C-terminus deletion (184 aa) was generated using specific primer set PF3-PR3Δ (Table 1) and replaced in place of wild type F3 fragment in pETmfd clone.

PCR amplification was conducted using specific primers sets at annealing temperatures of 53.5 58°C for 20 30 cycles.

To determine the LOS class in C. jejuni strains, we used specific primer sets for the classes A/B, C, D, and E, based on the DNA sequence of genes unique to the respective LOS locus class es) described earlier [9].

Amplification of the corresponding genes was achieved using specific primers and probe set and measured continuously using an ABI 7900 HTI Detection System.