Sentence examples for using specific primer combinations from inspiring English sources

Replacement-specific PCR amplifications of the corresponding SERA ser loci were generated using specific primer combinations.

Genotyping by PCR using specific primer combinations confirmed the desired integration events (Fig. S1).

Genotyping by PCR using specific primer combinations confirmed the expected recombination events (Fig. 5C).

We could indeed confirm the insertion at this position using specific primer combinations (Author response image 1B).

Integration-specific PCR amplification of the mCherry-tagged SERA1 or SERA2 was generated using specific primer combinations.

Genotyping by PCR using specific primer combinations confirmed the expected recombination events in sera1 and sera2 parasite lines (Fig. 5C), and RT-PCR demonstrated the complete absence of SERA1 transcripts and SERA2 transcripts in sera1 and sera2 blood stage parasites respectively (Fig. 5D).

Reactions were performed using specific primer for gene under study.

Inhibition of the enzymatic reactions involved in generating real-time qPCR signals from specific cDNA templates using specific primers, fluorogenic probes, or combinations of primers and fluorogenic probes can severely impact the precision of absolute and relative gene expression quantitative analysis.

Yet, this fragment could not be assigned to one of the other plasmids despite testing all combinations possible using specific primers for all linear plasmid ends.

Tos17 insertion sites were identified by PCR using specific primers for Tos17 (TAIL3 or T17-242R) in combination with the primers AOL71, AOL72, AOL356, or AOL357, which are specific for Pish, Npi37-1, Npi37-2, and/or Npi37-3 (Additional file 5).

RT-PCR amplification was obtained using specific primers.