In this work, whole Japropha curcas L. seed kernels were firstly treated in subcritical water (448 K, 2.0 MPa initial N2, 15 min, kernel to water ratio 0.5 g g−1) and then the treated kernels were used in the in situ production of biodiesel using a solvent mixture of 75% methanol and 25% acetic acid.
The crude product was purified by column chromatography (silica phase using a solvent mixture of NH4OH MeOH EA (1 4 15).
Cu2O nanoparticles are spherical (100 nm average size in diameter), while ZnO nanorods (~50 nm in width and 300 nm in length) can be prepared using a solvent mixture of 30 vol% of ethanol in water (Fig. 1).
Plasma metabolites were extracted using a solvent mixture of acetonitrile and methanol.
Chromatography was performed using a solvent mixture of ethyl acetate:methanol:25% ammoniumhydroxide solution at a volume ratio of 37.5 12.5 1.
After regular contact time interval (one hour), pesticide was extracted using equimolar solvent mixture of acetone and n-hexane.
The various lipid classes synthesized from fatty acids were then separated by TLC using the solvent mixture of hexane, ether, and acetic acid (80 : 20 : 3, vol/vol/vol), as previously described [ 19].
Using TLC, the polar lipid fraction was separated into PC, PS, PI, and PE using a solvent system consisting of chloroform:methanol:HAc boric acid [40 20 30 10 1.8g (v/v/v/v/w)] and neutral lipid fraction was separated into sterols, free fatty acids (FFAs), TG, and steryl esters using a solvent mixture of hexane:diethylether:formic acid [80 20 1 (v/v/v)].
In the HPLC analysis, Sia was derivatized using 1,2-diamino-4,5-methylenedioxybenzene 1,2-diamino-4,5-methylenedioxybenzene 1,2-diamino-4,5-methylenedioxybenzene 1,2-diamino-4,5-methylenedioxybenzene.6 mm, 5 μm; Vydaccordingria, CA, USA) using a solventomixture of acetonitrile/metHaral/watet (7 : 9 : 84), followed by identificalion based on the elution positions of standard Neu5Ac derivatives.
Subsequently, the lipids were separated by TLC on Silica Gel G60 plates [pre-treated with 1% potassium oxalate/2 mM EDTA in methanol/water (1 1, vol/vol)] using a solvent mixture of chloroform/acetone/methanol/glacial acetic acid/water (80 30 26:24:14, vol/vol) [ 23].
