Land cover products based on remote sensing imagery can indeed significantly be improved by using smarter algorithms, better timing of image acquisition, improved class definitions.
If so, the slow, inefficient health-care industry will enjoy faster transmission and smarter analysis of data, and better diagnoses.
For all samples, double-stranded cDNAs were synthesized from 1 μg of total RNA using SMARTer PCR cDNA synthesis kits (Clontech, Palo Alto, CA).
RACE ready cDNAs were generated using SMARTer RACE cDNA synthesis kit (Clontech).
Briefly, the methodology involves cDNA synthesis and amplification using SMARTer cDNA synthesis kit (TaKaRa BIO/Clontech, Mountain View, CA), normalization using Trimmer kit (Evrogen, Moscow, Russian Federation), fragmentation by sonication, end-polishing, ligation of adaptors, and amplification of the 454-ready sample.
Synthesis of full-length double-stranded cDNA (ds-cDNA) from total RNA was performed using SMARTer PCR cDNA Synthesis Kit (Clontech, CA, USA) according to the manufacturer's instructions, with the exception of using a modified CDS primer (5'-AAGCAGTGGTATCAACGCAGAGTAC GTGCAGTTTTTTTTTTTTTTTTVN-3').
Reverse transcription used SMARTer II A and 3' SMART CDS Primer II A oligonucleotides.
An editorial in The Washington Times last week praised El Al, the Israeli national airline, as employing the "smarter approach" of using "sophisticated intelligence analysis which allows them to predict which travelers constitute a possible threat and which do not".
Approximately 40 100 ng of total RNA was used to generate ∼10 μg of cDNA, using the SMARTer PCR cDNA synthesis kit (Clontech).
We used the 5′- and 3′-RACE analyses to determine the transcriptional initiation and termination site of CCAT1 using a SMARTer RACE cDNA Amplification Kit (Clontech, Palo Alto, CA, USA), according to the manufacturer's instructions.
