The overexpression was very obvious in the transgenic mice (compare Figures 2A and 2B) and could also be used to distinguish between transgenic and WT mice using small fragments of tail skin that were also used for PCR confirmation of the transgenic status.
In this study, we describe a simple and optimized protocol to expedite the production of highly enriched SSR libraries using small fragments of plant genomic DNA with different genome sizes.
The TaqMan Array is well suited to investigate the presence of these mechanisms in ovarian cancers alongside cellular chemosensitivity testing and clinical results, and its ability to use small fragments of tumour tissue makes it suited to development for future clinical use.
Since the downstream probe of the CpG island of p14ARF (Fig. 3, panel 3) was used, the smaller fragments of approximately 2.4 kb in size were produced.
Cooper and Penny looked at 16 orders of birds, using two small fragments of genes--600 base pairs of a protooncogene in the nucleus, and a 390-base pair region from the mitochondrion, a cellular organelle that has its own DNA.
It is noteworthy that the nodal support in previous studies, using only small fragments of DNA, was generally very poor throughout the estimated phylogenies (not shown), in contrast to the very robust support offered by complete mtDNA amino acid analysis.
Although preventing Fc receptor binding might help to reduce the background signals, the benefit of using small antibody fragments lacking the Fc portion like Fab, F ab 2, or single chain constructs for in vivo imaging is contradictory [32].
"What disturbed me most was the willingness to use very small fragments of information," he said, recounting how, over his six-month tour, he grew increasingly uneasy at what he saw.
Due to the deterioration of the surfaces and the inability to isolate paint layers, cross-sections were created using small samples of selected fragments, which were used to document the stratigraphies visually and with SEM/EDS.
Here, we focused on developing a methodology, which permits uniform amplification of a complex mixture of relatively short DNA fragments using small amounts of starting material.
