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Assays were performed to good laboratory practice (GLP) in a GLP-monitored laboratory using single batches of each antibody and reagent; incubations were rigorously controlled for temperature.
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The factor will be validated by applying it to analyses of several different experimental samples and measuring how close the reproducibility of these analyses are, using different batches of URR, relative to the reproducibility of the same analysis using a single batch of URR.
All samples were dispensed in one sequence over no more than 10 min using a single batch of cells in suspension.
After three successive glycosylation reactions using a single batch of the reconstituted vesicles and without changing the buffer, the acceptor was transformed into an O-glycan with chromatographic properties similar to glycans produced by C2GnT-I-expressing cells.
All studies were conducted using a single batch of ginger root extract.
We performed hybridizations to 8 microarrays using a single batch of LMW RNA prepared from mouse brain.
In this study, we proposed a combinatorial approach to interfere multiple WSSV gene expression using a single batch of dsRNA (hereafter called "multi-WSSV dsRNA").
Each HPV gene was expressed as GST-fusion proteins using a single batch of T7 reticulocyte lysate (Promega Corporation, Madison, WI, USA) per manufacturer's recommendations with 500 ng DNA.
Briefly, 25(OH)D2 and 25(OH)D3 samples were measured using tandem mass spectrometry, in 31 batches over a period of approximately 3 months [ 11], and 1,25(OH)2D samples were quantified by immunoassay [ 28] over a 2 months period using a single batch of reagents.
All experimental in vitro and in vivo results reported in the daptomycin no 1-study, also including the 107 clinical isolates tested for in vitro susceptibility, were recorded in 1991 using a single batch of quality-controlled daptomycin powder supplied by Ely Lilly (Giessen, Germany).
To increase performance and reproducibility, the use of single batches of reagents stored throughout the lifetime of individual monitoring projects is advisable.
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