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For comparison with fluorescence approaches, we began these studies by using similar protocols as those described previously [4], [5], and Ca2+-responses were recorded with whole brain bioluminescence imaging after a short bath application of KCl (70 mM) (Figure 1B-D, movie S1).
However, previous studies relying on the use of primary MSCs obtained using similar protocols as the one used here, although in different animal models, have shown that allogeneic MSCs may lose their suppressive effect in vivo and, actually, will elicit an immune response from the host [33], [34].
Purification of selenomethionyl MJ0927 were performed using similar protocols as established for the native protein.
Time-courses of desensitization/resensitization of receptor PLC signalling in response to UTP were assessed using similar protocols as those described above for myography experiments, however shorter agonist applications at lower concentrations (R1/ R2 10 µM UTP, 30 s; Rmax 100 µM UTP, 30 s) were sufficient to induce significant desensitization.
Experiment 2 ( effect of organic carbon levels on algal growth ) Based on the result of Experiment 1, glycerol as an organic carbon source was chosen to explore the growth response of I. galbana to different levels of glycerol in mixotrophy using similar protocols as those in Experiment 1.
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The other group was administrated with PBS using similar protocol as described above.
Two mutants, F976C and L983C, were not significantly affected by either MTSES or MTSET (e.g., Figure 3C), as recently demonstrated for Cys-less using similar protocols.
This could be dealt with using similar protocols to those that have been recommended for disciplinary cases in the BHA's Integrity Review carried out earlier this year.
All samples were incubated and harvested using similar protocols.
Mouse neonatal cardiomyocytes were prepared using similar protocol.
These initial studies were not designed to specifically address state-dependence, but all studies used similar protocols and recordings as controls (see Methods).
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