Sentence examples for using shRNAs that target from inspiring English sources

However, expression levels of these genes were not significantly affected when only MAML2 was knocked down using shRNAs that target the exon 1 of the MAML2 gene (shM2-B1 and shM2-C1) (Fig.  2d).

To explore the role of other HDACs in AML cells, we used shRNAs that target HDAC2, HDAC3, and HDAC8 to treat K562 cells (Supplementary Figure S3A).

We further investigated the possible requirement for caspase-1 in cell death by using shRNA that targeted caspase-1.

We demonstrate this principle using a "minipool" composed of shRNAs that target the genes encoding the reported protein targets of approved drugs.

We have achieved specific reduction of CYP3A4 expression using a set of three novel shRNAs that target the CYP3A4 coding region.

We used the Whitehead Institute siRNA selection program to design shRNAs that target multiple regions of Dali or Pou3f3.

PTEN knockdown in RAW 264.7 cells was performed using lentiviral transduction to stably express short hairpin RNAs (shRNAs) that target PTEN.

Moreover, cancer cells with suppression of CKIε expression using shRNA constructs that targeted the CKIε gene had reduced growth rates, a reduced capacity to migrate and reduced tumour growth in vivo (Fig 3).

Using a shRNA that targets the sequence GGAACGTCTTGGACATCAT, which is comparable to the one used for RAW264.7 murine macrophages [ 13] and identical, but independently designed, to the one used for MTLn3 rat mammary adenocarcinoma cells [ 11], we finally could generate a knock-down of CRN5, however, only in CT26 cells.

Consistent with the overall decrease in protrusion density using shRNA targeting EphB2, we found that EphB2 knockdown causes a reduction in both spine and filopodia density (Figure 3).

Previously we showed that Cdc42 is required for structural and functional plasticity of dendritic spines using shRNA targeted to Cdc42 in rat hippocampus (Murakoshi et al., 2011).