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These algorithm are initially trained with the multi-electrode array on a Personal Computer (PC) using several samples of waters (still, sparkling and tap) to obtain the optimum architecture of the networks.
Using several samples of the same material and testing them at the same strain rate and temperature, but different incremental strains, an accurate estimate of the material's isothermal flow stress can be obtained.
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Recent reports [ 28] on marked variations in aberration patterns within the same tumor is likely to increase the number of studies using several samples taken from each tumor.
The efficiency of the qPCR reactions (with all set of primers) was calculated using several dilutions of cDNA from biofilm samples.
We validated the microarray findings by quantifying irg1, ifit3, and socs3 transcripts via quantitative reverse transcription PCR (qRT-PCR) using several of the original RNA samples as templates.
The performance of the one-step mRT-PCR assay was evaluated and compared with previously published RT-qPCRs [ 13, 14] using several different types of field samples collected in ROK, including bovine serum, bovine whole blood, bovine brain, goat serum, and Culicoides biting midges.
Liquid samples were first outgassed using several iterations of freeze-pump-thaw cycles prior to their introduction into the interaction region through an inlet valve creating an effusive beam of gaseous sample.
The approach of using several loci and sampling individuals from a large distribution range to estimate a common level of LD may also have masked the variability of LD among genes that is now emerging as whole gene studies report high levels of LD in some of the genes examined (Lepoittevin et al. 2012; Namroud et al. 2010; Pavy et al. 2012).
In the last test we used as positive controls several samples of α-CEA displaying phages prepared earlier and conserved at 4°C for a long time (9 to 55 months).
Human CRC CSC/CIC have previously been isolated from CRC cell lines and primary CRC samples using several methods, including the use of cell surface markers (e.g. CD133), SP cells using Hoechst 33342 analysis and the Aldefluor assay.
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