Sentence examples for using several primer sets from inspiring English sources

Then, the sequences in the 5′-upstream, 3′-downstream, exon and intron were obtained by PCR using several primer sets (data not shown).

However, even though we used several primer sets, we did not amplify any chimeric transcript.

DNA samples obtained from frozen brain tissues were analyzed by nested PCR using several primer pairs.

The interaction between the DEXI promoter region and intron 19 of CLEC16A was confirmed using several different primer sets.

In determining the phylactic lineages of strain 1801/UPM and 1802/KB, several primer sets were used to amplify the 5.8 rRNA gene regions.

Several primer sets flanking the two probe target sites were tested to amplify the target region from case and control DNA samples using a long PCR protocol.

Robust SRF and TFAP2 binding was observed in HEK293 cells using several qRT-PCR primer sets (Figure 1B) encompassing the promoter region of FXN, while binding of SP1 was found to be far weaker than that of SRF or TFAP2 (Figure 1C).

The in-frame deletion of so2426 was confirmed by PCR amplification using several sets of primers (ISU2426/ISD2426, OP840, OP900, kanF/kanR in Table 3) and DNA sequencing.

The DGGE profile suggests low microbial diversity because even when using several sets of primers to amplify different regions of the 16S rRNA, few amplicons were obtained.

PCR analysis using several sets of primers within the Tol2-BAC and Southern blot analysis using the internal NheI sites revealed that no gross structural alterations of the Tol2-BAC occurred upon integration.

Further, we performed PCR analysis using several sets of primers within the Tol2-BAC and confirmed that no gross structural alterations of the Tol2-BAC occurred upon integration in 3A and 3B.