Sentence examples for using several primer combinations from inspiring English sources
For Berlinianche, sequences homologous to matR could not be obtained using several primer combinations.
Despite using several primer combinations, we could not demonstrate the presence of cellular and HPV junctions in any of the samples tested.
No GsAG1 transcripts were detected in the doubled-flower mutant by RT-PCR using several primer combinations (data not shown), and no truncated GsAG1 transcripts were detected by 3′-RACE.
A total of 65 samples that were antibody-positive for Sin Nombre or Laguna Negra virus by enzyme-linked immunosorbent assay were processed by nested reverse transcription polymerase chain reaction (RT-PCR) by using several primer combinations in the M and S genome segments.
We sequenced four mitochondrial genes: a portion of the 12S ribosomal RNA gene (12S, longest sequence of 833 bp), the complete ATP synthase F0 subunit 8 (ATP8, 168 bp) and subunit 6 (ATP684684 bp) genes, as well as the complete NADH dehydrogenase subunit 6 (ND6, 519 bp) using several primer pair combinations via polymerase chain reaction (PCR) (table 2).
DNA samples obtained from frozen brain tissues were analyzed by nested PCR using several primer pairs.
A 380 bp region of the histone gene was amplified and sequenced in both directions using the primers HexA-F and HexA-R [ 56]. 2,100 bp of 18 S and 2,450 bp of 28 S were amplified using several overlapping primer combinations [ 57].
Four strains Cx, Ch, C3 and C4, and their counterparts, four space flown strains Sx, Xh, S3 and S4, were analysed by amplified fragment length polymorphism (AFLP) with several primer combinations.
We report several primer combinations that might be of utility for informing relationships within and across groups in the Thelypodieae.
A hair sample (OMModCl, table 1) from a living muskox was amplified, products cloned, and multiple sequences determined using several of the primer combinations used in this study.
In S. leucichthys, the sdY gene was also PCR amplified from genomic DNA using several of the primer combinations that are listed in Tables 2 and 3.
