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The efficiency of the qPCR reactions (with all set of primers) was calculated using several dilutions of cDNA from biofilm samples.
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Only one dilution was used in this study because the little additional benefit expected from using several dilutions would not have outweighed the costs [ 18].
We investigated this question using various dilutions of several vaginal products (lubricants and spermicides) and the Abacus ABAcard for PSA detection.
We believe that the use of several dilutions of norepinephrine is of great clinical interest.
Several dilutions of CS were used to evaluate the effect of different concentrations in the system using dilution airflows of 0.1, 0.2, 0.5, 1.0, 1.5, 2.0, and 3.0 L/min, which corresponded to %CS and TPM concentrations of 69% =29.2 mg/L; 54% =22.2 mg/L; 32% =13.0 mg/L; 19% =7.7 mg/L; 13% =5.4 mg/L, 10%=4.22 mg/L, and 7%=2.9mg/L/L respectively.
RNA was quantified and quality assessed by running several dilutions of each sample using the Agilent RNA 6000 nano Kit and Agilent Bioanalyzer 2100.
To assess RNA quality and quantity, several dilutions of each sample were analysed using the Agilent RNA 6000 nano Kit and Agilent Bioanalyzer 2100.
A dose response chemotaxis assay with several dilutions of the conditioned media was performed.
Efficiency of amplifications was determined by running a standard curve with several dilutions of cDNA.
We prepared several dilutions of the mucomodulators employed (from 10-1 M to 10-8 M).
We first assessed transduction levels using serial dilutions of LNT.hrGFP.
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