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Studies using several cell lines with variable levels of adenoviral infectivity revealed that this enhanced effect is evident in the cells with poor infectivity to adenovirus.
To identify the cytotoxicity of ursolic acid, we first examined whether ursolic acid could inhibit the proliferation of CRC by using several cell lines including SW480, SW620, LoVo, and RKO.
Using several cell lines and human breast tissues, we showed that TRAF4 localizes to a remarkable subdomain of the plasma membrane corresponding to multiprotein complexes that form TJs. TJs are intercellular adhesion structures that are indispensable for correct epithelium organization.
By using several cell lines we will better be able to identify the genes critical for ES differentiation as these should be common for all three.
A recent study by Nagaraja and colleagues [ 39] describes gene expression patterns corresponding to normal breast, noninvasive breast cancer, and invasive breast cancer by using several cell lines.
This method has been tested using several cell lines and is preferred over traditional BrdU detection since it is more sensitive and allows multicolor and multiplex analysis in flow cytometry and imaging [ 3].
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In contrast, with TRT-L20, TEL-1, TERT and Ab-2 antibodies, no specific stained band was observed using several cell line lysates or from tumour samples.
We used several cell lines in our in vitro studies (Osipo et al, 2008); however, we chose to use BT474 ErbB-2-positive breast cancer cells as they are sensitive to trastuzumab and have been used numerous times in preclinical studies (Nagata et al, 2004; Rimawi et al, 2011) to represent the clinical setting.
Anti-inflammatory assays were performed using NO production by macrophage RAW 264.7 cell lines induced by LPS/IFN-g and cytotoxic activity was determined using several cancer cell lines and one normal cell line.
In an attempt to establish the identity of other possible receptors for the Opc protein, studies were undertaken using several distinct cell lines of human origin.
The cell-killing efficacy of ING1mini was tested using several cancer cell lines (MDA-MB-468, H1299, U-2 OS, Saos-2 and U-87 MG) representing different tumor types.
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