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Essentially, we evaluated the methylation levels of sequences obtained from bisulfite conversion of the tested primers using serial mixtures of DNA samples obtained from hippocampal tissues of two adult male mice, one from a saline-control litter and the other from a cocaine-treatment litter.
Analytical sensitivity was analyzed using serial mixtures of mutant and wild-type plasmid DNA.
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The analytical detection limit (sensitivity limit) of the MS-qLNAPCR assay was determined using the ABI7000™ instrument using serial dilution mixtures of SssI-treated DNA: untreated DNA (100%, 50%, 10%, 1%, 0.1%0.01%% each containing 1.5 μg of template DNA).
All primers were submitted to efficiency tests using serial dilutions of mixture of cDNAs from five melanoma cell lines (WM35, WM793, WM9, 1205, and WM1617).
A standard curve was generated for each gene using serial dilutions of a concentrated cDNA mixture to assess the amplification efficiency.
The linearity and sensitivity of the assay is evaluated using serial dilutions of single species and heterogeneous DNA mixtures.
We first assessed transduction levels using serial dilutions of LNT.hrGFP.
Standard curves were obtained using serial dilutions of control sample.
Values were quantified using serial dilutions of sodium nitrite.
A standard curve was prepared using serial dilutions of PGE2.
We investigate the three-dimensional structure of a model γ γ′ alloy using serial sectioning and digital reconstruction.
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