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Using sequence from the hookworm expressed sequence tag project, specific primers were designed and used to amplify Ac-api-1 from A. caninum infective L3 cDNA by PCR.
This was illustrated by the generation of a PWM using sequence from the RpZRE3-promoter-18 data-set (Figure 4), which revealed negligible sequence conservation outside of the core element.
When bovine genome coordinates were available, the 3' end of the 3' flanking sequence of each SNP was extended (using sequence from the bovine genome assembly) prior to performing the comparison with the human genome, to give a total flanking sequence length of 20 kbp.
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The phylogeography of Coreoperca whiteheadi was studied using sequences from the mitochondrial cytochrome b (cytb) gene and first intron of the S7 ribosomal protein gene.
To investigate the large-scale gene expression in different tea plant clones, a custom oligonucleotide microarray was developed using sequences from the RNA-seq study to design the probes because a commercial array is unavailable for the tea plant.
The same general topology was also found using sequences from the ENCODE consortium [18], [39].
Bayesian coalescent-based methods were used to estimate the substitution rate and the tMRCA, using sequences from the E1/E2 and NS5B genes.
Phylogenetic analyses were performed using sequences from the NS5B and E2 regions with the Maximum Likelihood, Neighbor Joining and Parsimony methods.
To this end, we mined our TSAs using sequences from the diatom Phaeodactylum tricornutum as queries.
As a positive control, the same methodology was implemented in Arabidopsis, using sequences from the 16 sRNA libraries available.
The identified polymorphic region was further analyzed at the nucleotide sequence level using sequences from the three human genomic sequence assemblies in the database.
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