To better understand the origins of substrate specificity within these two isoforms, we used sequence and structural analysis to predict residues and domains likely to be involved in conferring substrate specificity.
Using bioinformatics sequence and structural analysis we predict the existence of a StAR-related lipid-transfer (START) domain in pyst-a proteins.
A recent study, aimed at identifying polymorphic toxins in bacterial genomes using comparative analysis, sequence and structural analysis, identified RhsD as putative T6SS effectors of P. ananatis, based on the presence of PAAR repeats on this protein [ 59].
Using a combination of sequence and structural analysis, we analyzed protein-protein interactions in F1-ATPase, Sec23p/Sec24p, DNA-directed RNA polymerase and nuclear pore complexes, and found that interacting domain pair(s) for a given interaction exhibits higher level of co-evolution than the non-interacting domain pairs.
Using a bioinformatics approach based on both sequence and structural analysis, we have traced the evolution of this larger network in 26 organisms, from the uni-cellular ancestors of metazoans, through basal metazoans, to vertebrates.
In this study, we used sequence alignment, structural analysis and site-directed mutagenesis to examine the evolutionary relatedness of human noroviruses in terms of their interaction with the HBGA receptors.
Homologs of functionally characterized T6SS effectors such as type VI lipase effectors 1-5, type VI amidase 1-4, type VI secretion glycoside hydrolase effectors, Small secreted proteins 1-6 and Type VI secretion exported 1-2, were not identified in P. ananatis T6SS-2 gene cluster, using sequence analysis and structural homology search tools.
Protein sequence and structural analysis suggests that Dkks and colipases have the same disulfide-bonding pattern and a similar fold.
Introduction to computational biology emphasizing the fundamentals of nucleic acid and protein sequence and structural analysis, also including an introduction to the analysis of complex biological systems.
Nipa Chongdar, Saumya Dasgupta and Gautam Basu performed the sequence and structural analysis.
