All Western blots were repeated at least twice using separate cell culture preparations with similar results.
Both CBC8C (1 100) and anti-TCTEX1D4N (1:1000) antibodies were used in separate blots for immunodetection of protein expression.
Blots for P lethal hits were visualized with horseradish peroxidase-conjugated secondaries and enhanced chemiluminescence; total tau levels were measured using blots separate from those used for AT8.
For each sample, the average of two mean TLs calculated from two separate blots was used for statistical analysis.
Separate blots were used to probe for phosphorylated and total STAT1.
The results of Western blotting for phosphorylated STAT3 for these samples were from two separate blots.
After 48 h, cellular proteins were extracted and separated using Western blotting analysis.
Equal amounts of protein were separated using SDS-PAGE and blotted on nitrocellulose membranes (GE Healthcare, Munich, Germany).
Proteins of supernatant and CSK pellet were separated using SDS-PAGE and blotted on nitrocellulose membrane (GE Healthcare).
Proteins were loaded and separated using SDS-PAGE and blotted onto PVDF membranes.
