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Leaves from five virus-infected grapevines along with their respective controls were used separately and pigments estimated by two independent times using separate batches of tissue.
In the event that suspect taxa are still of interest, repeat sequencing should be carried out on additional samples using separate batches of DNA extraction kits/reagents, and, ideally, a non-sequencing-based approach (such as traditional culturing or FISH, using properly validated probe sets) should also be used to further confirm their presence in the samples.
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All studies were performed on 8 16 separate cultures for each measure and treatment, using 2 4 separate batches of cells.
Comparison of four independent DBD preparations using two separate batches of synthesized oligonucleotides for a reference dsDNA substrate shows that the standard deviation of the measured fluorescence anisotropy is less than 3 5%, smaller than the size point used in the figures.
The HPLC experiment was carried out using a separate batch of animals, which received IV injection of a single dose of free GEM, GEM LB-MSNP, and PTX/GEM LB-MSNP, as described in Figure 2 A. The total dose of GEM was 100 mg/kg per injection.
When we tested spatial memory performance in a Morris water maze task using a separate batch of animals, no differences were observed between APP/PS1 mice and wildtype littermates at 4 months of age; wildtype and transgenic animals performed equally well, both during the 5-day training and in the probe trial.
These experiments were carried out using the two separate batches of pretreated corn stover at the same pretreatment condition, and then averaged for the final data and error.
All studies were performed on 8 16 separate cultures for each measure and treatment, using two to four separate batches of cells.
Each study was performed using two to five separate batches of cells, with three or four independent cultures for each treatment in each batch; each batch of cells comprised a separately prepared, frozen and thawed passage.
Co-cultures were prepared using muscle cells from several separate batches of mRNA-injected embryos and neurons from normal embryos and in each preparation 3 6 coverslips were examined.
Two separate batches of seven monolithic columns were used for separation of the digest using a 7.5-min water-acetonitrile gradient at a flow rate of 2 μl/min.
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